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Sample GSM871713 Query DataSets for GSM871713
Status Public on Sep 01, 2012
Title tomato_OGDH_antisense_4
Sample type RNA
 
Source name leaf
Organism Solanum lycopersicum
Characteristics species: Solanum lycopersicum
variety: MoneyMaker;
tissue: leaf
age: 28 days
Extracted molecule total RNA
Extraction protocol TriZol extraction according to manufacturer (Invitrogen, Life Technologies GmbH, Darmstadt, Germany)
Label biotin
Label protocol Manufacturer standard protocol (Affymetrix)
 
Hybridization protocol Manufacturer standard protocol (Affymetrix)
Scan protocol Manufacturer standard protocol (Affymetrix)
Data processing MAS5 normalization as implemented in the GCOS analysis software provided by affymetrix. The data were further normalized using the RMA method (Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U,Speed TP. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics. 2003 Apr;4(2):249-64. PubMed PMID: 12925520.).
 
Submission date Feb 08, 2012
Last update date Sep 01, 2012
Contact name Marc Lohse
E-mail(s) lohse@mpimp-golm.mpg.de
Organization name MPIMP-Golm
Department Stitt
Lab Stitt
Street address Am Muehlenberg 1
City Potsdam
ZIP/Postal code 14476
Country Germany
 
Platform ID GPL4741
Series (1)
GSE35618 Molecular analysis of a tomato OGDH antisense line

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.164926875
AFFX-BioB-5_at 8.374559439
AFFX-BioB-M_at 8.572515124
AFFX-BioC-3_at 9.871883293
AFFX-BioC-5_at 9.757101369
AFFX-BioDn-3_at 12.11976538
AFFX-BioDn-5_at 11.00215233
AFFX-CreX-3_at 13.67678239
AFFX-CreX-5_at 13.49008906
AFFX-DapX-3_at 3.768846309
AFFX-DapX-5_at 3.52067891
AFFX-DapX-M_at 4.396867151
AFFX-Le_17SrRNA_at 6.291513414
AFFX-Le_25SrRNA_at 6.45950158
AFFX-Le_GlutTrans_3_at 11.01137716
AFFX-Le_GlutTrans_5_at 12.19127767
AFFX-Le_GlutTrans_M_r_at 10.21633578
AFFX-Le_PhyB2_3_at 6.72470095
AFFX-Le_PhyB2_5_at 3.695483398
AFFX-Le_PhyB2_M_at 3.696820809

Total number of rows: 10209

Table truncated, full table size 307 Kbytes.




Supplementary file Size Download File type/resource
GSM871713_Araujo_B4_20110722.CEL.gz 1.5 Mb (ftp)(http) CEL
GSM871713_Araujo_B4_20110722.CHP.gz 2.7 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
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