|
| Status |
Public on Aug 03, 2012 |
| Title |
input in Drosophila virilis |
| Sample type |
SRA |
| |
|
| Source name |
embryo
|
| Organism |
Drosophila virilis |
| Characteristics |
developmental stage: embryo 0-12h
fraction: input
custom antibody: none
|
| Treatment protocol |
no treatment
|
| Growth protocol |
flies are kept at room temperature
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Two grams of embryos were used for two chromatin preparations, sonicated 20 cycles (10s on / 30s off) on a BRANSON SONIFIER 250 with output control set at 1.5. Libraries were prepared with the Illumina TruSeq DNA Sample Preparation Kit
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
input in D. vir
|
| Data processing |
Alignment: Sequence reads were obtained and mapped to the each genome build using Bowtie 0.12.7 software using the default setting.
Genome Build:
dvir1_2-input.bed: dvir1.2 flybase
|
| |
|
| Submission date |
Feb 09, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Jingping Yang |
| E-mail(s) |
jpyang@nju.edu.cn
|
| Organization name |
Nanjing University
|
| Department |
Medical School
|
| Street address |
22 Hankou Road
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210000 |
| Country |
China |
| |
|
| Platform ID |
GPL13312 |
| Series (1) |
| GSE35648 |
ChIP-seq study of BEAF binding in four Drosophila spcies |
|
| Relations |
| SRA |
SRX119309 |
| BioSample |
SAMN00783963 |
