|
| Status |
Public on Feb 10, 2012 |
| Title |
Run2_D035 |
| Sample type |
RNA |
| |
|
| Source name |
iPSC-derived cardiomyocytes, Day 35
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPSC-derived cardiomyocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cell samples using TRIZol reagent according to the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Total RNA samples were converted to Cy3 labeled-cDNA using TotalPrep RNA amplification kit from Ambion.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the whole-genome gene expression direct hybridization protocol from Illumina.
|
| Scan protocol |
Beadchips were scanned using Illumina iScan, standard protocol.
|
| Description |
Replicate 2
|
| Data processing |
The data were log2 transformed and normalised using quantile normalization with Partek Genomics Suite software, version 6.5 (Partek, Inc., St. Louis, MO).
|
| |
|
| Submission date |
Feb 09, 2012 |
| Last update date |
Feb 10, 2012 |
| Contact name |
Morgane RAVON |
| Organization name |
Hoffmann-La Roche
|
| Street address |
Grenzacherstrasse 124
|
| City |
Basel |
| ZIP/Postal code |
4070 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6884 |
| Series (2) |
| GSE35671 |
Comparison of mRNA expression profiling of differentiating human-induced pluripotent stem cell (hiPSC)–derived cardiomyocytes, biopsies from fetal, adult and hypertensive human hearts and primary cardiomyocytes |
| GSE35673 |
Expression profiling analysis of differentiating human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes |
|
