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Sample GSM873448 Query DataSets for GSM873448
Status Public on Feb 16, 2012
Title Mono-LPS-3-2104
Sample type RNA
 
Source name Umbilical Cord blood Monocytes; LPS
Organism Homo sapiens
Characteristics tissue: Human umbilical cord blood
cell type: Monocytes
Treatment protocol Cells were pre-incubated with PBS (vehicle control) or equimolar concentrations (10-8 M) of IL-10 or DEX for 1 h, then stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
Growth protocol PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Qiagen RNeasy spin columns.
Label Biotin
Label protocol Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
 
Hybridization protocol Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
Scan protocol Chips were scanned by Gene Chip 3000 scanner
Description Gene Expression data from the Umbilical Cord Blood Monocytes treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
Data processing Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
 
Submission date Feb 09, 2012
Last update date Sep 01, 2016
Contact name Dennis Davidson
E-mail(s) dendavidson48@gmail.com
Phone 5164553609
Organization name The Feinstein Institute for Medical Research
Department Pediatrics
Lab Neonatology Research Lab
Street address 350 Community Dr
City Manhasset
State/province New York
ZIP/Postal code 11030
Country USA
 
Platform ID GPL570
Series (1)
GSE35683 Exogenous Interleukin-10 versus Glucocorticoids: Effect on Gene Expression and Pro-inflammatory Cytokine Release in Polymorphonuclear Leukocytes and Monocytes of the Newborn
Relations
Reanalyzed by GSE86362

Data table header descriptions
ID_REF
VALUE log2 GC-RMA

Data table
ID_REF VALUE
AFFX-BIOB-5_AT 6.88607
AFFX-BIOB-M_AT 7.75917
AFFX-BIOB-3_AT 6.52713
AFFX-BIOC-5_AT 7.97148
AFFX-BIOC-3_AT 8.75654
AFFX-BIODN-5_AT 10.2966
AFFX-BIODN-3_AT 11.0964
AFFX-CREX-5_AT 14.2417
AFFX-CREX-3_AT 14.0813
AFFX-DAPX-5_AT 4.69194
AFFX-DAPX-M_AT 8.12489
AFFX-DAPX-3_AT 11.1989
AFFX-LYSX-5_AT 5.21331
AFFX-LYSX-M_AT 5.55304
AFFX-LYSX-3_AT 8.26028
AFFX-PHEX-5_AT 4.16522
AFFX-PHEX-M_AT 5.72367
AFFX-PHEX-3_AT 7.14236
AFFX-THRX-5_AT 3.44552
AFFX-THRX-M_AT 5.22366

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM873448.CEL.gz 8.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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