|
| Status |
Public on Feb 16, 2012 |
| Title |
PMN-IL10-2-2093 |
| Sample type |
RNA |
| |
|
| Source name |
Umbilical Cord blood Polymorphonucler cells; LPS+IL-10
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human umbilical cord blood
cell type: Polymorphonuclear cells
|
| Treatment protocol |
Cells were pre-incubated with PBS (vehicle control) or equimolar concentrations (10-8 M) of IL-10 or DEX for 1 h, then stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
|
| Growth protocol |
PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen RNeasy spin columns.
|
| Label |
Biotin
|
| Label protocol |
Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
|
| |
|
| Hybridization protocol |
Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
|
| Scan protocol |
Chips were scanned by Gene Chip 3000 scanner
|
| Description |
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with 10^ -8 M Interleukin-10 for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Data processing |
Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
|
| |
|
| Submission date |
Feb 09, 2012 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Dennis Davidson |
| E-mail(s) |
dendavidson48@gmail.com
|
| Phone |
5164553609
|
| Organization name |
The Feinstein Institute for Medical Research
|
| Department |
Pediatrics
|
| Lab |
Neonatology Research Lab
|
| Street address |
350 Community Dr
|
| City |
Manhasset |
| State/province |
New York |
| ZIP/Postal code |
11030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE35683 |
Exogenous Interleukin-10 versus Glucocorticoids: Effect on Gene Expression and Pro-inflammatory Cytokine Release in Polymorphonuclear Leukocytes and Monocytes of the Newborn |
|
| Relations |
| Reanalyzed by |
GSE86362 |
