|
| Status |
Public on Mar 05, 2012 |
| Title |
V205G B |
| Sample type |
RNA |
| |
|
| Source name |
G1ME
|
| Organism |
Mus musculus |
| Characteristics |
strain: J1
cell type: G1ME
cell line: GATA1-deficient cell line with megakaryocyte and erythrocyte differentiation potential
protocol: Cells grown in TPO supernatant (10% v/v)
retrovirus: MigR1-HA-V205G
cell subtype: Sorted GFP-postive cells
|
| Treatment protocol |
Retroviral supernatant was added to the cells at 1e6 cells per ml, and the culture was centrifuged at 3200rpm for 90min. The cells were incuabted in the virus for an additional 4h, and then the virus was removed. The cells were gorwn in growth media for 68hours. GFP-positive cells were sorted on a Moflo cell sorter. The cells were allowed to recover for 4h in growth media, and then RNA was extracted.
|
| Growth protocol |
G1ME cells were cultured in 1% TPO supernatant (v/v)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy column (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Standard Illumina label protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 2
|
| Data processing |
Data were quantile normalized and background was subtracted with IlluminaGUI
|
| |
|
| Submission date |
Feb 10, 2012 |
| Last update date |
Mar 05, 2012 |
| Contact name |
Timothy Michael Chlon |
| E-mail(s) |
tchlon@u.northwestern.edu, timothy.chlon@cchmc.org, tchlon@gmail.com
|
| Organization name |
Cincinnati Children's
|
| Department |
Oncology
|
| Lab |
Wells
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE35695 |
The role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation |
| GSE35708 |
Cofactor-mediated Restriction of GATA-1 Chromatin Occupancy Coordinates Lineage-specific Gene Expression |
|
