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Status
Public on May 01, 2006
Title
Hoxa11, Hoxd11 null E13.5 kidney rep 2 (430B)
Sample type
RNA
Source name
Hoxa11, Hoxd11 null E13.5 kidneys replicate 2
Organism
Mus musculus Characteristics
Hoxa11, Hoxd11 double null targeted embryonic kidney at E13.5, mixed C57, C3H and CF1 strain, whole kidney
Extracted molecule
total RNA
Extraction protocol
Both kidneys were cleanly dissected from an E13.5 HoxA11/D11 targeted null embryo, rinsed in PBS, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label
biotin
Label protocol
30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Hoxa11, Hoxd11 double null targeted complete (whole) E13.5 kidney replicate 2
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 12, 2005
Last update date
Dec 12, 2005
Contact name
Kristopher Robert Schwab
E-mail(s)
kristopher.schwab@cchmc.org Organization name
Cincinnati Children's Hospital Research Foundation
Department
Developmental Biology
Lab
S. Steven Potter
Street address
3333 Burnett Ave
City
Cincinnati
State/province
OH
ZIP/Postal code
45229
Country
USA
Platform ID
GPL340 Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
2025.1
P
0.013811
AFFX-BioB-M_at
1967.8
P
0.000581
AFFX-BioB-3_at
923.2
P
0.018281
AFFX-BioC-5_at
3422.5
P
0.000754
AFFX-BioC-3_at
1705.5
P
0.000509
AFFX-BioDn-5_at
3580.6
P
0.000095
AFFX-BioDn-3_at
23095.2
P
0.000509
AFFX-CreX-5_at
29912.3
P
0.000044
AFFX-CreX-3_at
54291.5
P
0.000044
AFFX-DapX-5_at
566.9
M
0.054213
AFFX-DapX-M_at
637
M
0.058444
AFFX-DapX-3_at
46.4
A
0.9273
AFFX-LysX-5_at
44.6
A
0.760937
AFFX-LysX-M_at
114.3
A
0.58862
AFFX-LysX-3_at
257.1
A
0.250796
AFFX-PheX-5_at
35.1
A
0.843268
AFFX-PheX-M_at
36.6
A
0.941556
AFFX-PheX-3_at
439.3
A
0.216524
AFFX-ThrX-5_at
52.2
A
0.783476
AFFX-ThrX-M_at
142.4
A
0.39692
22575 618 Kbytes .Supplementary file Size Download File type/resource GSM87397.CEL.gz
3.5 Mb
(ftp) (http) CEL
GSM87397.EXP.gz
501 b
(ftp) (http) EXP
