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Status
Public on May 01, 2006
Title
Hoxa11, Hoxd11 null E16.5 kidney rep 1 (430B)
Sample type
RNA
Source name
Hoxa11, Hoxd11 null E16.5 kidneys replicate 1
Organism
Mus musculus Characteristics
Hoxa11, Hoxd11 double null targeted embryonic kidney at E16.5, mixed C57, C3H and CF1 strain, whole kidney
Extracted molecule
total RNA
Extraction protocol
Both kidneys were cleanly dissected from an E16.5 HoxA11/D11 targeted null embryo, rinsed in PBS, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label
biotin
Label protocol
30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Hoxa11, Hoxd11 double null targeted complete (whole) E16.5 kidney replicate 1
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 12, 2005
Last update date
Dec 12, 2005
Contact name
Kristopher Robert Schwab
E-mail(s)
kristopher.schwab@cchmc.org Organization name
Cincinnati Children's Hospital Research Foundation
Department
Developmental Biology
Lab
S. Steven Potter
Street address
3333 Burnett Ave
City
Cincinnati
State/province
OH
ZIP/Postal code
45229
Country
USA
Platform ID
GPL340 Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
2537.4
P
0.000581
AFFX-BioB-M_at
3700.7
P
0.000052
AFFX-BioB-3_at
1905.4
P
0.000225
AFFX-BioC-5_at
8680
P
0.000044
AFFX-BioC-3_at
8392
P
0.000052
AFFX-BioDn-5_at
16805.8
P
0.000044
AFFX-BioDn-3_at
30462.6
P
0.000052
AFFX-CreX-5_at
47873.5
P
0.000044
AFFX-CreX-3_at
86274.5
P
0.000044
AFFX-DapX-5_at
167.2
A
0.102165
AFFX-DapX-M_at
169.2
A
0.275146
AFFX-DapX-3_at
17.6
A
0.949771
AFFX-LysX-5_at
9.6
A
0.897835
AFFX-LysX-M_at
60
A
0.804734
AFFX-LysX-3_at
64.5
A
0.425962
AFFX-PheX-5_at
31
A
0.814869
AFFX-PheX-M_at
11.4
A
0.993813
AFFX-PheX-3_at
193.4
A
0.48511
AFFX-ThrX-5_at
49.7
A
0.824672
AFFX-ThrX-M_at
14.2
A
0.937071
22575 614 Kbytes .Supplementary file Size Download File type/resource GSM87398.CEL.gz
3.1 Mb
(ftp) (http) CEL
GSM87398.EXP.gz
489 b
(ftp) (http) EXP
