|
| Status |
Public on Feb 12, 2015 |
| Title |
PC-3_Exosomes_rep3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Exosomes from PC-3 cells
|
| Organism |
Homo sapiens |
| Characteristics |
sample source: Exosomes
|
| Treatment protocol |
PC-3 cells were incubated for 24 h under serum free conditions, and exosomes were isolated from conditioned media by ultracentrifugation.
|
| Growth protocol |
PC-3 cells were cultured in Ham’s F-12/ Dulbecco’s Modified Eagle’s Medium (1:1 mixture) with Glutamax supplemented with 7% foetal calf serum (FCS), 100 units/ml penicillin and 100 µg/ml streptomycin, in a humidified 5% CO2 atmosphere at 37 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using mirVana RNA isolation kit (Ambion) following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Exiqon protocol - miRCURY LNA microRNA Hi-Power Labeling. 750ng was labeled.
|
| |
|
| Channel 2 |
| Source name |
Total RNA from pooled sample 1-9
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using mirVana RNA isolation kit (Ambion) following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Exiqon protocol - miRCURY LNA microRNA Hi-Power Labeling. 750ng was labeled.
|
| |
|
| |
| Hybridization protocol |
Exiqon protocol – miRCURY LNA microRNA Array Instruction Manual, 6th generation arrays, hsa, mmu, rno. Tecan HS4800 hybridization station (Tecan, Austria)
|
| Scan protocol |
Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA). Ozone free environment
|
| Description |
Biological replicate 3 of 3. Exosomes isolated from cell culture media from PC-3 cells after 24 h incubation using ultracentrifugation.
|
| Data processing |
Image analysis by ImaGene® (miRCURY LNA microRNA Array Analysis Software, Exiqon, Denmark). Background correction using Normexp with an offset value of 10. No normalization. Median centration.
|
| |
|
| Submission date |
Feb 14, 2012 |
| Last update date |
Feb 12, 2015 |
| Contact name |
Nina Pettersen Hessvik |
| E-mail(s) |
nina.pettersen.hessvik@rr-research.no
|
| Organization name |
The Norwegian Radium Hospital, Oslo University Hospital
|
| Department |
Department for Biochemistry
|
| Street address |
Ullernchausseen 70
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL11434 |
| Series (1) |
| GSE35813 |
miRNA profile of exosomes released from PC-3 cells compared to parent cells |
|
