|
| Status |
Public on Mar 01, 2013 |
| Title |
Whole peripheral blood_Tempus Tube, Sample 13 |
| Sample type |
RNA |
| |
|
| Source name |
Whole peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: MAL
age: 60
percentage of body fat: 27.3
ethnicity: CAU
zip code: 30329
rna integrity score: 6.7
batch effect_date: 2010-07-22
plate id: 5455178033
chip_position: A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from Whole peripheral blood using Tempus Spin RNA Isolation kit. Quality control was performed using Agilent's Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Illumina's BeadArray protocol
|
| |
|
| Hybridization protocol |
Illumina's BeadArray protocol
|
| Scan protocol |
Illumina's BeadArray protocol and GenomeStudio (Gene expression Module)
|
| Description |
CHD1_000028
|
| Data processing |
The raw log2 transformed 14,343 probes that are expressed in the CHDWB study were normalized by ANOVA fitting technical effects of date of hybridization and RNA quality using JMP Genomics 5.0 (SAS Institute, Cary NC) and mean centered.
log2 transformed 14,343 probes expressed in CHDWB study, DATE and RIN3 effects removed, and mean-centered signal
|
| |
|
| Submission date |
Feb 15, 2012 |
| Last update date |
Mar 01, 2013 |
| Contact name |
Jinhee Kim |
| E-mail(s) |
jinhee.kim@northwestern.edu
|
| Organization name |
Northwestern University
|
| Department |
Infectious Disease
|
| Lab |
Martinelli
|
| Street address |
676 North St. Clair Street Arkes Suite 2330
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE35846 |
Blood-Informative Transcripts Define Nine Common Axes of Peripheral Blood Gene Expression |
|
