|
| Status |
Public on Jan 09, 2006 |
| Title |
2hr top replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
hypocotyl
|
| Organism |
Brassica oleracea |
| Characteristics |
wild type, 3 day old seedling
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 100 3-day-old B. oleracea seedlings that has been gravity stimulated were trisected into top flanks, vasculature, and bottom flanks. The top and bottom flanks were collected and Total RNA was extracted from the tissue using an RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Approximately 20 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
wild type, gravity treated Brassica hypocotyl tissue
|
| Data processing |
GCOS 3.0
|
| |
|
| Submission date |
Dec 14, 2005 |
| Last update date |
Jan 09, 2006 |
| Contact name |
Emmanuel Liscum |
| E-mail(s) |
liscume@missouri.edu
|
| Phone |
573-882-2672
|
| Fax |
573-884-9676
|
| URL |
http://www.biosci.missouri.edu/liscum/liscumlabpage.html
|
| Organization name |
University of Missouri
|
| Department |
Biological Sciences
|
| Lab |
Life Sciences Center
|
| Street address |
Rollin Rd
|
| City |
Columbia |
| State/province |
MO |
| ZIP/Postal code |
65211 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE3847 |
Tropic-stimulus induced changes in gene expression |
|
