|
| Status |
Public on Feb 23, 2012 |
| Title |
TG mouse lung, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
TG mouse lung
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mouse lung upper left lobe
strain: C57BL/6
genotype/variation: CC10-rtTA-IL13 transgenic (TG)
age: 6-10 weeks old mouse
|
| Treatment protocol |
Mice were given doxycycline-treated (final concentration 2 mg/mL) water for 7 days. After 7 days, mice were euthanized and the left upper lobes of the lungs were removed for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip Mouse miRNA Array. GeneChips were washed and stained according to standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned according to standard protocol.
|
| Description |
miRNA expression data from mouse lung upper left lobe
|
| Data processing |
The raw expression data were first processed for background estimation and correction, normalization, and summarization using miRNAQCTools, an Affymetrix software specifically developed for the GeneChip miRNA arrays.
|
| |
|
| Submission date |
Feb 21, 2012 |
| Last update date |
Feb 23, 2012 |
| Contact name |
Aik T Ooi |
| Organization name |
UCLA
|
| Department |
Pediatrics
|
| Street address |
650 Charles E Young Dr S, Factor 9-240
|
| City |
Los Angeles |
| ZIP/Postal code |
CA |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE35980 |
MicroRNA expression data from IL13-induced allergic airway inflammation of mice lungs |
| GSE37085 |
Expression and MeDIP microarray data from IL13-induced allergic airway inflammation of mice lungs |
|
