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Sample GSM878553 Query DataSets for GSM878553
Status Public on Feb 23, 2012
Title WT mouse lung, biological rep2
Sample type RNA
Source name WT mouse lung
Organism Mus musculus
Characteristics tissue: mouse lung upper left lobe
strain: C57BL/6
genotype/variation: wildtype
age: 6-10 weeks old mouse
Treatment protocol Mice were given doxycycline-treated (final concentration 2 mg/mL) water for 7 days. After 7 days, mice were euthanized and the left upper lobes of the lungs were removed for RNA extraction.
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip Mouse miRNA Array. GeneChips were washed and stained according to standard Affymetrix protocol.
Scan protocol GeneChips were scanned according to standard protocol.
Description miRNA expression data from mouse lung upper left lobe
Data processing The raw expression data were first processed for background estimation and correction, normalization, and summarization using miRNAQCTools, an Affymetrix software specifically developed for the GeneChip miRNA arrays.
Submission date Feb 21, 2012
Last update date Feb 23, 2012
Contact name Aik T Ooi
Organization name UCLA
Department Pediatrics
Street address 650 Charles E Young Dr S, Factor 9-240
City Los Angeles
ZIP/Postal code CA
Country USA
Platform ID GPL8786
Series (2)
GSE35980 MicroRNA expression data from IL13-induced allergic airway inflammation of mice lungs
GSE37085 Expression and MeDIP microarray data from IL13-induced allergic airway inflammation of mice lungs

Data table header descriptions
VALUE Processed microarray expression data in log2.

Data table
hsa-miR-1826_st 9.71777
mml-miR-127_st 4.158712
rno-miR-146b_st 5.586901
ptr-miR-127_st 4.757525
hsa-miR-127-3p_st 4.554352
age-miR-127_st 4.722392
ppy-miR-127_st 4.677145
bta-miR-127_st 4.916674
mmu-miR-685_st 4.872
lla-miR-127_st 4.911865
bta-miR-532_st 4.59082
rno-miR-324-5p_st 5.149901
rno-miR-324-3p_st 4.247938
hsa-miR-502-3p_st 4.215477
mmu-miR-379_st 3.866928
hsa-miR-146b-5p_st 4.452357
mml-miR-21_st 4.327025
age-miR-21_st 3.958961
mmu-miR-699_st 4.065849
mmu-miR-362-5p_st 4.190431

Total number of rows: 7625

Table truncated, full table size 183 Kbytes.

Supplementary file Size Download File type/resource
GSM878553.CEL.gz 139.5 Kb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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