|
Status |
Public on Feb 24, 2012 |
Title |
Saracatinib resistant PIK3CA mutant rep1 |
Sample type |
RNA |
|
|
Source name |
Resistant, PIK3CA mutant rep1
|
Organism |
Homo sapiens |
Characteristics |
tissue: Human colorectal tumor xenografted in six-week-old female athymic nude mice genotype: PIK3CA mutant resistant to saracatinib
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
Gene expression data from baseline, before treatment
|
Data processing |
The data were analyzed with RMA using Affymetrix Power Tools.
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|
|
Submission date |
Feb 22, 2012 |
Last update date |
Feb 25, 2012 |
Contact name |
Aik Choon Tan |
E-mail(s) |
AikChoon.Tan@HCI.utah.edu
|
Organization name |
Huntsman Cancer Institute
|
Department |
Oncological Sciences
|
Street address |
2000 Circle of Hope
|
City |
Salt Lake City |
State/province |
UT |
ZIP/Postal code |
84112 |
Country |
USA |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE36006 |
Common PIK3CA mutants and a novel 3’UTR mutation are associated with increased sensitivity to saracatinib |
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