|
| Status |
Public on Feb 24, 2012 |
| Title |
Saracatinib resistant PIK3CA mutant rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Resistant, PIK3CA mutant rep1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human colorectal tumor xenografted in six-week-old female athymic nude mice
genotype: PIK3CA mutant resistant to saracatinib
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from baseline, before treatment
|
| Data processing |
The data were analyzed with RMA using Affymetrix Power Tools.
|
| |
|
| Submission date |
Feb 22, 2012 |
| Last update date |
Feb 25, 2012 |
| Contact name |
Aik Choon Tan |
| E-mail(s) |
AikChoon.Tan@HCI.utah.edu
|
| Organization name |
Huntsman Cancer Institute
|
| Department |
Oncological Sciences
|
| Street address |
2000 Circle of Hope
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE36006 |
Common PIK3CA mutants and a novel 3’UTR mutation are associated with increased sensitivity to saracatinib |
|
