|
| Status |
Public on Mar 01, 2012 |
| Title |
dH1f-untreated-OSKM-biorep2 |
| Sample type |
RNA |
| |
|
| Source name |
Untreated dH1f fibroblasts, superinfected with OSKM retroviruses
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: dH1f fibroblasts
retroviral vector: Oct4, Sox2, Klf4 and c-Myc (OSKM)
treatment: none
|
| Treatment protocol |
dH1f fibroblasts were superinfected or not with retroviral vectors expressing Oct4, Sox2, Klf4 and c-Myc for 6 days. Fibroblasts were then treated or not with 10uM Dot1L inhibitor for 5 days.
|
| Growth protocol |
Fibroblasts were grown in alpha-MEM containing 10% inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from culture plates for each cell type using an RNeasy Mini kit coupled with an RNase-free DNase set (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Scanned using an Affymetrix HT Scanner.
|
| Description |
Untreated dH1f fibroblasts 6 days post-infection with retroviral vectors expressing Oct4, Sox2, Klf4 and c-Myc.
|
| Data processing |
The data were RMA-normalized using the open-source R-project statistical software with Bioconductor packages.
|
| |
|
| Submission date |
Feb 24, 2012 |
| Last update date |
Mar 01, 2012 |
| Contact name |
Tamer T Onder |
| Organization name |
Children's Hospital Boston
|
| Street address |
One Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE29253 |
Gene expression changes upon Dot1L knockdown and Dot1L inhibitor treatment during reprogramming |
|
