|
| Status |
Public on Feb 28, 2015 |
| Title |
Protoplast reprogramming_48h |
| Sample type |
SRA |
| |
|
| Source name |
P. patens protoplast cultures at 48 hours
|
| Organism |
Physcomitrium patens |
| Characteristics |
tissue: protoplast
time (culture): 48h
|
| Growth protocol |
samples taken from P. patens protoplast cultures at 0, 24 48 and 72 hours after protoplastes isolated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Illumina HiSeq 2000 DGE
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Adaptor tags, low quality tags and tags of copy number=1 were filtered,then the distinct clean tags were aligned to the reference P.patens database by Bowtie. The P.patens reference to which our clean tags were aligned was downloaded from JGI database (http://www.jgi.doe.gov/). The genome build version: V7.0, and the FTP download address is ftp://ftp.jgi-psf.org/pub/JGI_data/phytozome/v7.0/Ppatens/annotation/.
Genome Build:
48h.GeneExpression.txt: JGI v7.0
|
| |
|
| Submission date |
Feb 28, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Lihong Xiao |
| E-mail(s) |
xiaolihong6290@gmail.com
|
| Organization name |
Capital Normal University
|
| Street address |
No.105 North Road of West 3rd-ring Road.Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100048 |
| Country |
China |
| |
|
| Platform ID |
GPL14798 |
| Series (1) |
| GSE36117 |
Transcriptome of Protoplasts Reprogrammed into Stem Cells in Physcomitrella patens |
|
| Relations |
| SRA |
SRX122681 |
| BioSample |
SAMN00792924 |
