|
| Status |
Public on Oct 02, 2012 |
| Title |
DU145 parental sensitive 2 |
| Sample type |
RNA |
| |
|
| Source name |
DU145 parental Docetaxel-sensitive cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DU145
cell type: prostate cancer cells
phenotype: docetaxel sensitive
|
| Growth protocol |
Parental Docetaxel-sensitive and resistant cells were grown with RPMI supplemented with 10% FBS alone or under exposure to Docetaxel, respectively, before harvesting total RNA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix Human U133 Plus 2.0 or Affymetrix Human U133A 2.0 arrays.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
DU145 2
Gene expression data from DU145 parental Docetaxel-sensitive cells.
|
| Data processing |
The data were analyzed with Microarray Suite, MicroDB, and Data Mining tool software (Affymetrix). Quantile normalization and RMA summarization were used to obtain probeset level expression.
|
| |
|
| Submission date |
Feb 28, 2012 |
| Last update date |
Oct 02, 2012 |
| Contact name |
Josep Domingo-Domenech |
| E-mail(s) |
josep.domingo-domenech@mssm.edu
|
| Phone |
212-241-9145
|
| Organization name |
Mount Sinai School of Medicine
|
| Department |
Pathology
|
| Street address |
Annenberg Building, Room 24-16
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE36135 |
Expression data from prostate cancer Docetaxel-resistant cells |
|
