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Sample GSM886845 Query DataSets for GSM886845
Status Public on Mar 20, 2012
Title 786-O
Sample type RNA
 
Source name ATCC
Organism Homo sapiens
Characteristics primary site: kidney
histology: carcinoma
histology subtype1: clear_cell_renal_cell_carcinoma
Treatment protocol None
Growth protocol Cells lines were cultured following growth recommendations from the cell line source vendors. Cells were incubated at 37 ºC at 5% CO2 until 70% confluency was reached. Pellets were harvested 48 hours post media change, flash frozen, and stored at -80 ºC until nucleic acid extraction.
Extracted molecule total RNA
Extraction protocol RNA was isolated from frozen cell pellets, containing an average cell count of 4.5 million cells, via Trizol (Invitrogen) extraction following the manufacturers instructions. Samples were quantified using both the Nanodrop 8000 spectrophotometer (ThermoScientific) and via Agilent 2100 Bioanalyzer.
Label Biotin
Label protocol The Affymetrix One-Cycle Target labeling assay was used to synthesize hybridization target using 1 mg of total RNA. Labeled cRNA hybridization target was produced using a 3-day, semi-automated workflow
 
Hybridization protocol Fragmentation of the biotin-labeled cRNA prepared the sample for hybridization on the GeneChip Human U133 2.0 (PN 900467). The washing and staining was performed on the GeneChip Fluidics station using standard Affymetrix protocols outlined in the Expression Analysis Technical Manual, 2001, Affymetrix.
Scan protocol The GeneChip® Scanner 3000 was used for scanning the arrays.
Description Gene expression data from the CCLE
Data processing The data were analyzed with 2.14.0, using the packages affyio_1.22.0 and affy_1.32.0, and RMA as normalization method. rma() was used with default options (normalize=TRUE, background=TRUE) and with the package hgu133plus2hsentrezgcdf_15.0.0 from Brainarray for the CDF file.
 
Submission date Mar 06, 2012
Last update date Sep 01, 2016
Contact name Nicolas Stransky
E-mail(s) stransky@broadinstitute.org
URL http://www.broadinstitute.org/ccle
Organization name Broad Institute
Department Cancer Program
Lab Levi Garraway
Street address 7 Cambridge center
City Cambridge
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL15308
Series (2)
GSE36133 Expression data from the Cancer Cell Line Encyclopedia (CCLE)
GSE36139 SNP and Expression data from the Cancer Cell Line Encyclopedia (CCLE)
Relations
Reanalyzed by GSE86362

Data table header descriptions
ID_REF
VALUE RMA log2 signal intensity

Data table
ID_REF VALUE
1_at 4.7121
10_at 4.0844
100_at 8.0051
1000_at 10.475
10000_at 7.3755
100009676_at 5.8836
10001_at 8.4485
10002_at 3.849
10003_at 4.0474
10004_at 4.5049
100048912_at 4.43
100049716_at 4.4013
10005_at 8.1652
10006_at 8.8486
10007_at 8.9541
10008_at 4.1214
10009_at 7.9051
100093630_at 11.162
1001_at 5.9451
10010_at 9.1245

Total number of rows: 18926

Table truncated, full table size 294 Kbytes.




Supplementary file Size Download File type/resource
GSM886845.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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