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Status
Public on Apr 08, 2006
Title
Proloferation at 2 hours (A)
Sample type
RNA
Source name
HMVEC
Organism
Homo sapiens Characteristics
HMVECs induced with VEGF
Extracted molecule
total RNA
Extraction protocol
1.35E6 HMVEC cells were plated onto gelatin–coated plates and the cells were allowed to attach for 2 hours. Cells were collected by scraping, washed with PBS, and RNA prepared using Trizol (Invitrogen) extraction followed by 1:1 phenol/chloroform and ethanol precipitation. RNA integrity was monitored using an Agilent 2100 Bioanalyzer.
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
HMVEC induced with VEGF
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 21, 2005
Last update date
Mar 10, 2006
Contact name
suneeta mandava
E-mail(s)
smandava@anl.gov Organization name
Argonne National Lab
Department
Bio
Lab
Rodi
Street address
9700 S Cass ave
City
Argonne
State/province
IL
ZIP/Postal code
60439
Country
USA
Platform ID
GPL96 Series (1)
GSE3891 Endothelial Cell Morphogenesis
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
166.8
P
0.012547
AFFX-BioB-M_at
163.2
P
0.00844
AFFX-BioB-3_at
83.8
M
0.050229
AFFX-BioC-5_at
290.1
P
0.000258
AFFX-BioC-3_at
282
P
0.000972
AFFX-BioDn-5_at
351.2
P
0.000225
AFFX-BioDn-3_at
2111.3
P
0.00034
AFFX-CreX-5_at
2753.6
P
0.000044
AFFX-CreX-3_at
4931.6
P
0.000044
AFFX-DapX-5_at
19
A
0.340661
AFFX-DapX-M_at
17.4
A
0.425962
AFFX-DapX-3_at
4.4
A
0.897835
AFFX-LysX-5_at
19.7
A
0.5
AFFX-LysX-M_at
20.3
A
0.749204
AFFX-LysX-3_at
12.5
A
0.5
AFFX-PheX-5_at
4.4
A
0.814869
AFFX-PheX-M_at
7.2
A
0.824672
AFFX-PheX-3_at
13
A
0.772364
AFFX-ThrX-5_at
8.4
A
0.760937
AFFX-ThrX-M_at
5.9
A
0.834139
22283 592 Kbytes .Supplementary data files not provided
