|
Status |
Public on Aug 13, 2015 |
Title |
mouse glioma [12-05-11-4(30.2)] |
Sample type |
RNA |
|
|
Source name |
brain
|
Organism |
Mus musculus |
Characteristics |
strain: Balb/c cell type: glioma
|
Treatment protocol |
Initial DNA plasmid injection was the only treatment administered to mice
|
Growth protocol |
Mice injected at birth with DNA plasmids as described above developed spontaneous gliomas, which were harvested at day 50-60 post injection
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted usign RNEasy Qiagen kit according to manufacturer's instructions
|
Label |
Biotin
|
Label protocol |
The Ambion WT Expression Kit with accompanying protocol was used to label the samples
|
|
|
Hybridization protocol |
The Affymetrix Genechip WT Terminal Labeling and Hybridization protocol was used
|
Scan protocol |
Chips were scanned using the Affymetrix 7G Plus scanner and Command Console Software
|
Description |
Gene expression data from mouse brain
|
Data processing |
Data was processed by importing CEL files into Partek Software and RMA normalization was performed
|
|
|
Submission date |
Mar 07, 2012 |
Last update date |
Aug 13, 2015 |
Contact name |
Saleena Ghanny |
E-mail(s) |
ghannysa@njms.rutgers.edu
|
Organization name |
Rutgers University
|
Street address |
185 South Orange Ave
|
City |
Newark |
ZIP/Postal code |
07103 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE36337 |
Cytomegalovirus promotes maintenance and growth of glioblastoma stem cells [Mouse gene expression] |
GSE56752 |
Human Cytomegalovirus in Glioblastoma Stemness--Results from Human, Mouse and HCMV DNA arrays |
|