|
| Status |
Public on Apr 01, 2012 |
| Title |
delta-env1_cellulose_DD_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
T. reesei delta-env1 grown on cellulose in darkness
|
| Organism |
Trichoderma reesei |
| Characteristics |
background strain: QM9414
genotype/variation: delta-env1 (reduced growth rate in light, expression induced in light)
condition: darkness
|
| Treatment protocol |
Mycelium was harvested under red-safety light when cultivated in darkness and frozen in liquid nitrogen.
|
| Growth protocol |
Trichoderma reesei delta-blr1, delta-blr2 and delta-env1 strains were grown in 1 l Erlenmeyer flasks on a rotary shaker (200 rpm) at 28°C in Mandels-Andreotti minimal medium with 1% microcrystalline cellulose as carbon source in constant light (LL) or constant darkness (DD) for 72 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted as described in Tisch et al., 2011 (New insights into the mechanism of light modulated signaling by heterotrimeric G-proteins: ENVOY acts on gna1 and gna3 and adjusts cAMP levels in Trichoderma reesei (Hypocrea jecorina). Fungal Genet Biol, 48 (6): 631 – 40) with supplies provided by the RNeasy Plant Mini Kit (QIAGEN, Hilden, Germany). The quality of the RNA was controlled with the Experion Automated Electrophoresis System (Bio-Rad, Hercules, USA). Total RNA was treated with DNase (Fermentas, Vilnius, Lithuania) and purified using the RNeasy Mini Kit (QIAGEN). cDNA synthesis was done with the RevertAid H- First Strand cDNA Synthesis Kit (Fermentas) and Oligo-d(T) Primer.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
Trichoderma reesei
delta-env1_DD_rep1
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Mar 12, 2012 |
| Last update date |
Apr 01, 2012 |
| Contact name |
Doris Tisch |
| Organization name |
Technical University of Vienna
|
| Department |
Chemical Engineering
|
| Lab |
Biochemistry
|
| Street address |
Gumpendorferstraße 1a
|
| City |
Vienna |
| State/province |
Vienna |
| ZIP/Postal code |
1060 |
| Country |
Austria |
| |
|
| Platform ID |
GPL10642 |
| Series (1) |
| GSE36448 |
Expression analysis of Trichoderma reesei QM9414 and delta-blr1, delta-blr2 and delta-env1 in light and darkness |
|
