|
| Status |
Public on Mar 14, 2012 |
| Title |
CD4 memory T cells_0_hour_Young-3 |
| Sample type |
RNA |
| |
|
| Source name |
CD4 memory T cells_0_hour_Young
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD4 memory T cells
age: 20-35 years
|
| Treatment protocol |
T cells were stimulated with myeloid dendritic cells and TSST
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the Rneasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was obtained using the BioArray HighYield RNA Transcript Labeling Kit (Enzo).
|
| |
|
| Hybridization protocol |
10 ug cRNA were hybridized for 16 hr at 45 degree and 60 rpm to Human U133A1 Arrays in a hybridization oven 640 (Affymetrix). GeneChips were washed and stained using the Affymetrix Fluidics450 station.
|
| Scan protocol |
GeneChips were scanned using a seventh-generation GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from CD4 memory T cells before stimulation
|
| Data processing |
The CEL/CHP files were analyzed using Affymetrix default analysis settings and the Rosetta Resolver System.
|
| |
|
| Submission date |
Mar 13, 2012 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Mingcan Yu |
| Phone |
(650)723-9027
|
| Fax |
(650)721-1251
|
| Organization name |
Stanford University School of Medicine
|
| Department |
Medicine: Immunology and Rheumatology
|
| Lab |
Dr. Goronzy lab
|
| Street address |
CCSR Bldg. Rm. 2215, 269 Campus Drive West
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5166 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE36476 |
Gene expression in CD4 memory T cell responses with age |
|
| Relations |
| Reanalyzed by |
GSE86363 |
