|
Status |
Public on Mar 14, 2012 |
Title |
CD4 memory T cells_40_hour_Old-5 |
Sample type |
RNA |
|
|
Source name |
CD4 memory T cells_40_hour_Old
|
Organism |
Homo sapiens |
Characteristics |
cell type: CD4 memory T cells age: 70-75 years
|
Treatment protocol |
T cells were stimulated with myeloid dendritic cells and TSST
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was purified using the Rneasy Mini Kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was obtained using the BioArray HighYield RNA Transcript Labeling Kit (Enzo).
|
|
|
Hybridization protocol |
10 ug cRNA were hybridized for 16 hr at 45 degree and 60 rpm to Human U133A1 Arrays in a hybridization oven 640 (Affymetrix). GeneChips were washed and stained using the Affymetrix Fluidics450 station.
|
Scan protocol |
GeneChips were scanned using a seventh-generation GeneChip Scanner 3000 (Affymetrix).
|
Description |
Gene expression data from CD4 memory T cells at 40 hours after stimulation
|
Data processing |
The CEL/CHP files were analyzed using Affymetrix default analysis settings and the Rosetta Resolver System.
|
|
|
Submission date |
Mar 13, 2012 |
Last update date |
Sep 01, 2016 |
Contact name |
Mingcan Yu |
Phone |
(650)723-9027
|
Fax |
(650)721-1251
|
Organization name |
Stanford University School of Medicine
|
Department |
Medicine: Immunology and Rheumatology
|
Lab |
Dr. Goronzy lab
|
Street address |
CCSR Bldg. Rm. 2215, 269 Campus Drive West
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5166 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE36476 |
Gene expression in CD4 memory T cell responses with age |
|
Relations |
Reanalyzed by |
GSE86363 |