|
| Status |
Public on Mar 31, 2015 |
| Title |
Hypoxia-adapted/4% O2, larva, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Hypoxia-adapted/4% O2, larva
|
| Organism |
Drosophila melanogaster |
| Characteristics |
developmental stage: Third instar larva
|
| Treatment protocol |
Hypoxia-adapted Drosophila melanogaster were grown in chambers maintained at 4% O2 or reverted to 21% O2 for 5 generations, and generation-matched control flies were maintained in chambers at 21% O2. Cultures were visually inspected and flies were collected from three developmental stages: third instar larvae, 0.5-3 hr post-eclosion adults, and 7-9 day old adults.
|
| Growth protocol |
Flies were cultured on standard media and maintained in chambers adjusted to 4% or 21% O2, depending on experimental group.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from whole flies homogenized in TRIzol (Invitrogen, Carlsbad, CA) followed by a clean-up with RNeasy kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA.
|
| |
|
| Hybridization protocol |
10 ug of fragmented cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 7G Scanner.
|
| Description |
Gene expression data from 25 control third instar larvae adapted to and maintained in 4% O2
|
| Data processing |
Data were analyzed using the Plier algorithm implemented in the Affymetrix Expression Console software using the default settings.
|
| |
|
| Submission date |
Mar 14, 2012 |
| Last update date |
Mar 31, 2015 |
| Contact name |
Merril Gersten |
| E-mail(s) |
mgersten@ucsd.edu
|
| Organization name |
UCSD
|
| Street address |
9500 Gilman Drive #0412
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE36507 |
Gene expression in hypoxia-tolerant Drosophila melanogaster |
|
