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Status |
Public on Mar 20, 2013 |
Title |
Control knockdown, biological rep1 |
Sample type |
RNA |
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Source name |
MCF-7 cell with scamble KD for 72 hours
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Organism |
Homo sapiens |
Characteristics |
cell line: MCF-7 treatment: control KD for 72 hours
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Treatment protocol |
CtBP mRNA targetting oligos and the control oligos are from Dharmacon. Dharmafect1 transfection method was applied to introduce gene knockdown oligoes into the cells following manufacturer's manual.
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Growth protocol |
MCF-7 cells were maintained in regular DMEM supplemented with 10% (v/v) FBS, penicillin-streptomycin (Invitrogen) and insulin.
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Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNAeasy kit was applied to extract total RNA from MCF-7 cells following manufacturer's manual.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Data processing |
The data were analyzed with Gene Console using Affymetrix default analysis settings. RMA normalization.
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Submission date |
Mar 15, 2012 |
Last update date |
Mar 20, 2013 |
Contact name |
Lijun Di |
E-mail(s) |
dil@mail.nih.gov
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Organization name |
NCI
|
Street address |
41 center dr
|
City |
bethesda |
ZIP/Postal code |
20892 |
Country |
USA |
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Platform ID |
GPL570 |
Series (2) |
GSE36529 |
Expression data from CtBP knockdown MCF-7 cells |
GSE36598 |
Global transcriptional role of CtBP in breast cancer cells |
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