|
Status |
Public on Apr 11, 2012 |
Title |
M5616-liver-Chronic, 460971_A03 |
Sample type |
RNA |
|
|
Source name |
Woodchuck liver, chronic
|
Organism |
Marmota monax |
Characteristics |
tissue: liver whv infection status: chronic sample identifier: M5616
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from woodchuck liver tissues using the QIAamp RNA Blood Mini Kit (Qiagen Inc., Valencia, CA).
|
Label |
Cy3
|
Label protocol |
10 μg of total RNA was reverse-transcribed using the SuperScript double-stranded cDNA synthesis kit (Invitrogen, Inc., Calsbad, CA). After precipitation, double-stranded cDNA was labelled with Cy3 by using the Roche NimbleGen One-color DNA Labelling Kit (Roche NimbleGen, Inc., Madison, WI).
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|
|
Hybridization protocol |
Woodchuck Gene Expression HX3 Microarrays were hybridized with 6.7 μg Cy3-labelled cDNA for 16-20 hours at 42ºC and were washed and dried according to the manufacturer’s instructions.
|
Scan protocol |
Microarrays were scanned using a Roche NimbleGen MS200 Microarray Scanner at 2 μm pixel resolution. Features were extracted using NimbleScan Software.
|
Description |
460971_A03 mRNA
|
Data processing |
The gene expression data was normalized by the Robust Multichip Average (RMA) algorithm implemented in Partek Genomics Suite 6.5 (Partek, St. Louis MO).
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|
|
Submission date |
Mar 15, 2012 |
Last update date |
Apr 11, 2012 |
Contact name |
Donavan Cheng |
E-mail(s) |
donavan.cheng@gmail.com
|
Organization name |
Hoffmann-LaRoche
|
Department |
Translational Research Sciences
|
Street address |
340 Kingsland Ave
|
City |
Nutley |
State/province |
NJ |
ZIP/Postal code |
07110 |
Country |
USA |
|
|
Platform ID |
GPL15354 |
Series (2) |
GSE36250 |
Transcriptomic analysis of the woodchuck model of chronic hepatitis B |
GSE36533 |
Transcriptomic analysis of the woodchuck model of chronic hepatitis B: Liver gene expression in uninfected, resolved and chronically infected woodchucks |
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