|
| Status |
Public on May 01, 2012 |
| Title |
Caf40-ChIP |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Caf40 ChIP
|
| Organism |
Drosophila melanogaster |
| Characteristics |
fraction: Chromatin DNA
antibody: anti-FLAG
antibody vendor: Sigma
|
| Treatment protocol |
anti-FLAG antibody (Sigma)
|
| Growth protocol |
Drosophila cornmeal
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ModEncode ChIP: Biological samples were homogenized in 1.8% of formaldehyde (for cross-linking). The cross-linked chromatin was sonicated to an average size of 500bp.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Channel 2 |
| Source name |
Caf40 input
|
| Organism |
Drosophila melanogaster |
| Characteristics |
fraction: input
|
| Treatment protocol |
anti-FLAG antibody (Sigma)
|
| Growth protocol |
Drosophila cornmeal
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ModEncode ChIP: Biological samples were homogenized in 1.8% of formaldehyde (for cross-linking). The cross-linked chromatin was sonicated to an average size of 500bp.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| |
| Hybridization protocol |
Tecan HS 4800 Pro Hybridization Station
|
| Scan protocol |
High-throughput Genome Analysis Core (HGAC) in the Argonne National Laboratory, Affymetrix protocol
|
| Description |
Dm2
|
| Data processing |
Affy TAS
|
| |
|
| Submission date |
Mar 16, 2012 |
| Last update date |
May 01, 2012 |
| Contact name |
Sidi Chen |
| E-mail(s) |
chensidi@mit.edu
|
| Phone |
7734144158
|
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
Sharp
|
| Street address |
77 Mass Ave, 76-461
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL11397 |
| Series (2) |
| GSE36573 |
Zeus Caf40 ChIP-chip |
| GSE36920 |
Reshaping of global gene expression networks and sex-biased gene expression by integration of a nascent gene |
|
