|
| Status |
Public on Jun 25, 2012 |
| Title |
wild type fetal liver #3 |
| Sample type |
RNA |
| |
|
| Source name |
E14.5 Rnaseh2b wild type embryo
|
| Organism |
Mus musculus |
| Characteristics |
age: E14.5
line: Rnaseh2b
tissue: embryo
genotype/variation: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed by the acid guanidinium thiocyanate-phenol-chloroform method (Chomczynski, P. & Sacchi, N. The single-step method of RNA isolation by acid guanidiniumthiocyanate-phenol-chloroform extraction: twenty-something years on. Nat. Protoc. 1, 581-585(2006).)
|
| Label |
biotin
|
| Label protocol |
RNA was reverse transcribed, amplified and biotin-labeled using the Message Amp II-Biotin Enhanced Kit (Ambion)
|
| |
|
| Hybridization protocol |
Hybridization, staining and scanning of the GeneChip Mouse Genome 430 2.0microarrays were performed according to the Affymetrix GeneChip Expression Analysis Technical Manual P/N 702232 Rev. 3 (Affymetrix)
|
| Scan protocol |
according to the Affymetrix GeneChip Expression Analysis Technical Manual P/N 702232 Rev. 3 (Affymetrix)
|
| Data processing |
Microarray data was analysed using the Genespring GX software
|
| |
|
| Submission date |
Mar 21, 2012 |
| Last update date |
Jun 25, 2012 |
| Contact name |
Björn Hiller |
| E-mail(s) |
bjoern.hiller@tu-dresden.de
|
| Phone |
00493514586503
|
| Organization name |
TU Dresden
|
| Department |
Institute for Immunology
|
| Lab |
AG Roers
|
| Street address |
Fiedlerstrasse 42
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE36687 |
Microarray of Rnaseh2b KOF and RnaseH2b wild type fetal liver |
|
