|
Status |
Public on Jun 25, 2012 |
Title |
KOF fetal liver #3 |
Sample type |
RNA |
|
|
Source name |
E14.5 Rnaseh2b KOF embryo
|
Organism |
Mus musculus |
Characteristics |
age: E14.5 line: Rnaseh2b tissue: embryo genotype/variation: KOF
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extraction was performed by the acid guanidinium thiocyanate-phenol-chloroform method (Chomczynski, P. & Sacchi, N. The single-step method of RNA isolation by acid guanidiniumthiocyanate-phenol-chloroform extraction: twenty-something years on. Nat. Protoc. 1, 581-585(2006).)
|
Label |
biotin
|
Label protocol |
RNA was reverse transcribed, amplified and biotin-labeled using the Message Amp II-Biotin Enhanced Kit (Ambion)
|
|
|
Hybridization protocol |
Hybridization, staining and scanning of the GeneChip Mouse Genome 430 2.0microarrays were performed according to the Affymetrix GeneChip Expression Analysis Technical Manual P/N 702232 Rev. 3 (Affymetrix)
|
Scan protocol |
according to the Affymetrix GeneChip Expression Analysis Technical Manual P/N 702232 Rev. 3 (Affymetrix)
|
Data processing |
Microarray data was analysed using the Genespring GX software
|
|
|
Submission date |
Mar 21, 2012 |
Last update date |
Jun 25, 2012 |
Contact name |
Björn Hiller |
E-mail(s) |
bjoern.hiller@tu-dresden.de
|
Phone |
00493514586503
|
Organization name |
TU Dresden
|
Department |
Institute for Immunology
|
Lab |
AG Roers
|
Street address |
Fiedlerstrasse 42
|
City |
Dresden |
ZIP/Postal code |
01307 |
Country |
Germany |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE36687 |
Microarray of Rnaseh2b KOF and RnaseH2b wild type fetal liver |
|