|
| Status |
Public on Apr 08, 2012 |
| Title |
BTX623 2hr sucrose repeat I |
| Sample type |
RNA |
| |
|
| Source name |
BTX623 2hr Repeat I
|
| Organism |
Sorghum bicolor |
| Characteristics |
tissue: whole seedling
treatment: 5% sucrose
time: 2h
cultivar: BTX623
age: Two-week old
|
| Treatment protocol |
The two-week old seedlings of grain (BTX623) and sweet (Keller) sorghum lines were subjected to 5% of sucrose solution, and samples were then collected in 0, 2, and 6 h intervals respectively.
|
| Growth protocol |
Both grain and sweet sorghum (Sorghum bicolor L. Moench cultivars BT×623 and Keller were used for all experiments in this study. Their seeds were imbibed in water. After germination, they were planted in greenhouse and were grown under natural light and temperature conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were prepared using RNeasy Plant mini kit (Qiagen). Total RNA quality was analyzed by nanodrop reading and Agilent Bioanalyzer running
|
| Label |
Cy3
|
| Label protocol |
labeled according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
Gene expression after 2hr in 5% sucrose solution
|
| Data processing |
Data processed with FeatureExtraction v9.5
|
| |
|
| Submission date |
Mar 21, 2012 |
| Last update date |
Apr 08, 2012 |
| Contact name |
Shu-Ye Jiang |
| Organization name |
Temasek Life Sciences Laboratory, National University of Singapore
|
| Street address |
1 Research Link
|
| City |
Singapore |
| ZIP/Postal code |
117604 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL15369 |
| Series (1) |
| GSE36689 |
Sorghum gene expression using Agilent custom 4x44K Microarray |
|
