|
| Status |
Public on Aug 03, 2012 |
| Title |
SAMPLE 99_miRNA_Normal [FF] |
| Sample type |
RNA |
| |
|
| Source name |
miRNA_FF_normal
|
| Organism |
Homo sapiens |
| Characteristics |
tissue origin: uninvolved lung
sample type: fresh-frozen (FF) normal tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the miRNeasy kit (Qiagen Inc, Valencia, California, USA) using the manufacturer’s protocols. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared from 100-200ng total RNA using the DASL Assay (cDNA-mediated annealing, selection, extension and ligation)
|
| |
|
| Hybridization protocol |
Fluorescently labelled cDNA PCR products were hybridized to the Illumina Sentrix BeadChip according to the manufacturer's protocol
|
| Scan protocol |
Scanned using the Illumina Bead Array ReaderÂ
|
| Description |
SAMPLE 99
|
| Data processing |
The data were normalized using quantile normalization with BeadStudio software. The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Mar 23, 2012 |
| Last update date |
Aug 03, 2012 |
| Contact name |
Jin Sung Jang |
| Organization name |
Mayo Clinic
|
| Street address |
200 First St SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE36681 |
Increased miR-708 expression in NSCLC and its association with poor survival in lung adenocarcinoma from Never Smokers |
|
