|
| Status |
Public on Jun 17, 2013 |
| Title |
PB Patient 3 |
| Sample type |
RNA |
| |
|
| Source name |
purified CD4+ T cells from breast cancer patient 3
|
| Organism |
Homo sapiens |
| Characteristics |
source tissue: peripheral blood
cell type: purified CD4+ T cells
disease state: breast cancer
|
| Treatment protocol |
no treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner.
|
| Description |
Gene expression data from purified CD4+ T cells from patient blood at T0
CD4+ T cells were positively purified from patient samples or healthy donor blood using Dynabeads CD4 (Invitrogen).
|
| Data processing |
The data (CEL files) were analyzed in R version 2.3.0 for RMA normalization.
|
| |
|
| Submission date |
Mar 23, 2012 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Chunyan GU |
| E-mail(s) |
chunyan.gu@bordet.be
|
| Phone |
+32(0)25413711
|
| Organization name |
Free University of Brussels
|
| Department |
Medecine (Jules Bordet Institute)
|
| Lab |
Molecular Immunology
|
| Street address |
Bld de Waterloo 127
|
| City |
Brussels |
| ZIP/Postal code |
1000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE36765 |
Gene expression profiling of CD4+ T cells infiltrating human breast cancer (Discovery Set) |
| GSE36769 |
CD4+ TIL in human breast cancer |
|
| Relations |
| Reanalyzed by |
GSE86362 |
