|
| Status |
Public on Jun 17, 2013 |
| Title |
Total CD4 NS + SN034 at 24h |
| Sample type |
RNA |
| |
|
| Source name |
purified CD4+ T cells from a healthy donor (+ 24h treatment with SN from breast cancer patient 034)
|
| Organism |
Homo sapiens |
| Characteristics |
source tissue: peripheral blood
cell type: CD4
disease state: healthy
treatment: + 24h treatment with SN from breast cancer patient 034
|
| Treatment protocol |
Cells (2x106 cells/ml) were incubated in X-VIVO 20 (Lonza) overnight following isolation. Cell viability was controlled the following day by flow cytometry (>95%) and then an equal volume of tumor SN (diluted; concentrations normalized to total RNA quantity of non-CD4 cells in each tumor) or X-VIVO was added.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (3' IVT Express Kit Manual, 2009, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner.
|
| Description |
Gene expression data from total CD4+ T cells from healthy donor blood treated with SN for 24h
Total CD4+ T cells were negatively purified from a healthy donor (D5) blood using CD4+ T Cell Isolation Kit II (Myltenyi Biotec).
|
| Data processing |
The data (CEL files) were analyzed in R version 2.5.1 for RMA normalization (together with 9 other samples of the same experiments which are not included in this study).
|
| |
|
| Submission date |
Mar 23, 2012 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Chunyan GU |
| E-mail(s) |
chunyan.gu@bordet.be
|
| Phone |
+32(0)25413711
|
| Organization name |
Free University of Brussels
|
| Department |
Medecine (Jules Bordet Institute)
|
| Lab |
Molecular Immunology
|
| Street address |
Bld de Waterloo 127
|
| City |
Brussels |
| ZIP/Postal code |
1000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE36768 |
Effect of human breast tumor supernatant on normal CD4+ T cells (Preliminary Experiment) |
| GSE36769 |
CD4+ TIL in human breast cancer |
|
| Relations |
| Reanalyzed by |
GSE86362 |
