|
| Status |
Public on Jan 10, 2013 |
| Title |
wild type shoot, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
shoot from 7-day-old wild type seedlings grown on vertical plates
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia-0
genotype/variation: wild type
tissue: shoot
age: 7 days
|
| Growth protocol |
Arabidopsis thaliana Columbia-0 ecotype (Col0) and the root meristemless1 (rml1) mutant were grown on vertical plates containing PANG2 medium (1 mM KNO3; 150 µM CaCl2; 100 µM NaH2PO4; 20 µM MgSO4, micronutrients; 0.5 g.L-1 0.5 % sucrose and MES buffer pH 5.7) under an irradiance of 150 µmol*m-2*s-1 with a 16 h photoperiod and a temperature of 20ºC with 50 % relative humidity for 7 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Plants of the rml1 and Columbia-0 ecotype were harvested, separated into roots and shoots, immediately frozen in liquid nitrogen and stored at -80ºC until RNA extraction was performed. Total RNA was extracted from frozen shoots and roots using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol.
|
| Label |
Biotin
|
| Label protocol |
Labeling was performed as described in the Agronomics1 Standard Operating Procedures.
|
| |
|
| Hybridization protocol |
Hybridization was performed as described in the Agronomics1 Standard Operating procedures.
|
| Scan protocol |
Scanning was performed as described in the Agronomics1 Standard Operating procedures.
|
| Description |
WT.S3
Columbia-0 replicate comprised 30 shoots.
|
| Data processing |
The Robust Multiarray Averaging (RMA) method of microarray normalization and summarization was used.
|
| |
|
| Submission date |
Mar 28, 2012 |
| Last update date |
Jan 10, 2013 |
| Contact name |
Pedro Diaz-Vivancos |
| E-mail(s) |
pdv@cebas.csic.es
|
| Organization name |
CEBAS-CSIC
|
| Department |
Plant Breeding
|
| Street address |
Campus Universitario de Espinardo, 25
|
| City |
Murcia |
| ZIP/Postal code |
30100 |
| Country |
Spain |
| |
|
| Platform ID |
GPL14926 |
| Series (1) |
| GSE36893 |
Redox regulation of auxin and strigolactone signaling in the control of Arabidopsis thaliana root growth |
|
