|
| Status |
Public on Apr 17, 2013 |
| Title |
3xTg-AD-h, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus of 3xTg-hetero mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Hippocampus
genotype: 3xTg-hetero
age: 14 months of age
Sex: male
|
| Treatment protocol |
Brains were removed from each mouse under pentobarbital anesthesia (i.p), with perfusion of 40 ml of saline via the left ventricle. Hippocampi were isolated and preserved at –80°C until RNA preparation.
|
| Growth protocol |
Each mouse was maintained in SPF condition and sacrificed at 14 months of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using a combination of Isogen (Nippon Gene) and the RNeasy Mini Kit (Qiagen), according to the manufacturers’ instructions.
|
| Label |
biotin
|
| Label protocol |
The Ambion WT Expression Kit and the GeneChip WT Terminal Labeling and Controls Kit (Affymetrix) were used to generate amplified and biotinylated sense-strand DNA targets from expressed transcripts (100 ng of total RNA).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 µg of cDNA were hybridized for 17 hr at 45℃ on Affymetrix Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 7G.
|
| Data processing |
CEL files were imported into Affymetrix Expression Console Software, and CHP files were generated.
|
| |
|
| Submission date |
Apr 02, 2012 |
| Last update date |
Apr 17, 2013 |
| Contact name |
Yusaku Nakabeppu |
| E-mail(s) |
yusaku@bioreg.kyushu-u.ac.jp
|
| Phone |
81-92-642-6800
|
| Organization name |
Medical Institute of Bioregulation, Kyushu University
|
| Department |
Department of Immunobiology and Neuroscience
|
| Lab |
Division of Neurofunctional Genomics
|
| Street address |
3-1-1 Maidashi Higashi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE36981 |
Expression data from Alzheimer's disease model mouse |
|
