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Sample GSM908120 Query DataSets for GSM908120
Status Public on Mar 04, 2013
Title E176_04_Leptin_4_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_04_Leptin_4
treatment: Leptin
weeks of treatment: 4
subject: E176_04
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.70043199
10001_at 5.156227163
10002_at 2.716935842
10003_at 2.498205568
100048912_at 2.248836467
10004_at 3.347728426
10005_at 5.08138823
10006_at 7.325195551
10007_at 4.56021948
10009_at 2.306768788
1000_at 2.842825358
10010_at 6.398810866
100126791_at 3.340274932
100128124_at 2.732884031
100128640_at 2.866283421
100129128_at 2.424855437
100129250_at 6.265276621
100129271_at 2.87952626
100129482_at 4.642825018
100129973_at 2.459002149

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908120_Holly_05.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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