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Sample GSM908127 Query DataSets for GSM908127
Status Public on Mar 04, 2013
Title E176_08_Placebo_1_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_08_Placebo_1
treatment: Placebo
weeks of treatment: 1
subject: E176_08
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.666151502
10001_at 5.810186773
10002_at 2.668787324
10003_at 2.574553738
100048912_at 2.808624522
10004_at 3.37578648
10005_at 4.894028824
10006_at 7.236744504
10007_at 4.354769326
10009_at 2.707228776
1000_at 2.688416144
10010_at 6.078453856
100126791_at 3.568877001
100128124_at 2.504612473
100128640_at 3.386490518
100129128_at 2.38662907
100129250_at 6.373731917
100129271_at 2.889510218
100129482_at 5.046818275
100129973_at 2.802560449

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908127_Holly_12.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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