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Sample GSM908135 Query DataSets for GSM908135
Status Public on Mar 04, 2013
Title E176_14_Leptin_3_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_14_Leptin_3
treatment: Leptin
weeks of treatment: 3
subject: E176_14
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.983937164
10001_at 5.589609982
10002_at 2.52636531
10003_at 2.520865381
100048912_at 2.29528655
10004_at 3.579396848
10005_at 4.974583075
10006_at 7.327471638
10007_at 5.585054009
10009_at 2.313461874
1000_at 2.708876484
10010_at 6.241925898
100126791_at 3.37246236
100128124_at 2.830725767
100128640_at 2.884352142
100129128_at 2.146074297
100129250_at 6.150765942
100129271_at 3.003879733
100129482_at 4.096930965
100129973_at 2.280907129

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908135_Holly_21.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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