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Sample GSM908146 Query DataSets for GSM908146
Status Public on Mar 04, 2013
Title E176_20_Leptin_1_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_20_Leptin_1
treatment: Leptin
weeks of treatment: 1
subject: E176_20
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.836650604
10001_at 5.443195395
10002_at 2.456570591
10003_at 2.472506394
100048912_at 2.678158363
10004_at 3.568853418
10005_at 5.017123751
10006_at 7.172183937
10007_at 5.563966297
10009_at 2.293459274
1000_at 2.600414319
10010_at 5.973868946
100126791_at 3.518912818
100128124_at 2.632472008
100128640_at 2.867811167
100129128_at 2.121720635
100129250_at 6.000419946
100129271_at 2.890018294
100129482_at 4.998104289
100129973_at 2.422933774

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908146_Holly_32.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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