|
| Status |
Public on Apr 28, 2012 |
| Title |
Colo829 infected with BANCR shRNA 2 |
| Sample type |
RNA |
| |
|
| Source name |
Colo829 melanoma cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Colo829 melanoma cells
genotype/variation: BANCR knockdown
|
| Treatment protocol |
Cells were lentivirally infected with either SCR control shRNA or independent shRNAs targeting BANCR
|
| Growth protocol |
Colo829 cells were grown in RPMI 1640 medium supplemented with 10% FCS and antibiotics
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kits were used to isolate total RNA.
|
| Label |
biotin
|
| Label protocol |
Affymetrix 3' IVT Express Kit
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization, Wash, and Stain Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
shRNA 2
|
| Data processing |
RMA normalization using justRMA package in R 2.11.1
|
| |
|
| Submission date |
Apr 10, 2012 |
| Last update date |
Oct 11, 2022 |
| Contact name |
Douglas Porter |
| Organization name |
Stanford
|
| Department |
Dermatology
|
| Lab |
Khavari
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE37132 |
Oncogenic BRAFV600E remodels the melanocyte transcriptome and induces BANCR to regulate melanoma cell migration [Affymetrix] |
| GSE37169 |
Oncogenic BRAFV600E remodels the melanocyte transcriptome and induces BANCR to regulate melanoma cell migration |
|
