|
Status |
Public on May 01, 2012 |
Title |
Caf40-RNAi-whiteprepupae2 |
Sample type |
RNA |
|
|
Source name |
Caf40-RNAi-whiteprepupae2
|
Organism |
Drosophila melanogaster |
Characteristics |
tissue: white prepupa genotype/variation: Caf40
|
Treatment protocol |
RNAi
|
Growth protocol |
Drosophila cornmeal
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol-Qiagen
|
Label |
Cy5
|
Label protocol |
two-color Quick Amp labeling Kit
|
|
|
Hybridization protocol |
Tecan HS 4800 Pro Hybridization Station
|
Scan protocol |
Agilent Technologies Scanner G2505C US80900103
|
Description |
US80900103_251897210233_S01_GE2_107_Sep09_1_3.txt Biological replicate
|
Data processing |
Agilent Feature Extraction Software, Bioconductor package marray function backgroundCorrect with method option normexp for background subtraction; function normalizewithArrays with method option loess for within array normalization ; function normalizebetweenArrays with method option Aquantile for between array normalization, All FLAG data points treated as NA (null).
|
|
|
Submission date |
Apr 13, 2012 |
Last update date |
May 01, 2012 |
Contact name |
Sidi Chen |
E-mail(s) |
chensidi@mit.edu
|
Phone |
7734144158
|
Organization name |
MIT
|
Department |
Biology
|
Lab |
Sharp
|
Street address |
77 Mass Ave, 76-461
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02139 |
Country |
USA |
|
|
Platform ID |
GPL6385 |
Series (2) |
GSE36574 |
Zeus RNAi expression profiling |
GSE36920 |
Reshaping of global gene expression networks and sex-biased gene expression by integration of a nascent gene |
|