|
| Status |
Public on May 01, 2012 |
| Title |
wildtype-wholebody3 |
| Sample type |
RNA |
| |
|
| Source name |
wildtype-wholebody3
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: wholebody
genotype/variation: wildtype
|
| Treatment protocol |
RNAi
|
| Growth protocol |
Drosophila cornmeal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol-Qiagen
|
| Label |
Cy3
|
| Label protocol |
two-color Quick Amp labeling Kit
|
| |
|
| Hybridization protocol |
Tecan HS 4800 Pro Hybridization Station
|
| Scan protocol |
Agilent Technologies Scanner G2505C US80900103
|
| Description |
US80900103_251897210153_S01_GE2_105_Dec08_1_2.txt
Biological replicate
|
| Data processing |
Agilent Feature Extraction Software, Bioconductor package marray function backgroundCorrect with method option normexp for background subtraction; function normalizewithArrays with method option loess for within array normalization ; function normalizebetweenArrays with method option Aquantile for between array normalization, All FLAG data points treated as NA (null).
|
| |
|
| Submission date |
Apr 13, 2012 |
| Last update date |
May 01, 2012 |
| Contact name |
Sidi Chen |
| E-mail(s) |
chensidi@mit.edu
|
| Phone |
7734144158
|
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
Sharp
|
| Street address |
77 Mass Ave, 76-461
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL6385 |
| Series (2) |
| GSE36574 |
Zeus RNAi expression profiling |
| GSE36920 |
Reshaping of global gene expression networks and sex-biased gene expression by integration of a nascent gene |
|
