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Sample GSM915758 Query DataSets for GSM915758
Status Public on Apr 17, 2012
Title BM_CD34_TBO2249
Sample type RNA
 
Source name Normal BMCD34
Organism Homo sapiens
Characteristics diagnosis: healthy control
cell type: Cryopreserved MNCs
Extracted molecule total RNA
Extraction protocol Total RNA extracted using TRIzol reagent (Invitrogen, Carlsbad, CA)according to manufacturer's protocol.
Label Biotin
Label protocol Target was labeled with Enzo BioArray High Yield RNA Trascript Labeling Kit (Enzo Life Technologies, Farmingdale, NY) according to manufacturer's protocol.
 
Hybridization protocol Hybridization was performed according to Affymetrix protocol.
Scan protocol Arrays were scanned according to Affymetrix protocol.
Description GSM239340
0BM_CD34_23_F_TBO2249
Cryopreserved MNCs
Data processing .DAT files were generated using GCOS 1.2.1 software (Affymetrix). CEL files were generated using MAS 5.0 softare (Affymetrix) with target signals for probe sets scaled to 500. Model-based expression levels were computed from .CEL files using dChip software after normalization by invariant set method.
 
Submission date Apr 16, 2012
Last update date Apr 17, 2012
Contact name Sebastian K Ochsenreither
Organization name Charite Berlin
Department Hematology/Oncology
Street address Hindenburgdamm 30
City Berlin
State/province Berlin
ZIP/Postal code 12203
Country Germany
 
Platform ID GPL96
Series (1)
GSE37307 Aberrant expressed genes in AML
Relations
Reanalysis of GSM239340

Data table header descriptions
ID_REF
VALUE linear normalized signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 359.1
AFFX-BioB-M_at 364.41
AFFX-BioB-3_at 354.93
AFFX-BioC-5_at 765.87
AFFX-BioC-3_at 924.75
AFFX-BioDn-5_at 1015.17
AFFX-BioDn-3_at 1930.48
AFFX-CreX-5_at 5052.44
AFFX-CreX-3_at 4308.36
AFFX-DapX-5_at 19.21
AFFX-DapX-M_at 27.61
AFFX-DapX-3_at 12.22
AFFX-LysX-5_at 7.76
AFFX-LysX-M_at 11.8
AFFX-LysX-3_at 7.24
AFFX-PheX-5_at 5.89
AFFX-PheX-M_at 14.56
AFFX-PheX-3_at 61.74
AFFX-ThrX-5_at 6.31
AFFX-ThrX-M_at 16.51

Total number of rows: 22283

Table truncated, full table size 370 Kbytes.




Supplementary file Size Download File type/resource
GSM915758_0BM_CD34_23_F_TBO2249.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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