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Sample GSM915764 Query DataSets for GSM915764
Status Public on Apr 17, 2012
Title PB_DON040
Sample type RNA
 
Source name Normal PB
Organism Homo sapiens
Characteristics diagnosis: healthy control
cell type: Cryopreserved MNCs
Extracted molecule total RNA
Extraction protocol Total RNA extracted using TRIzol reagent (Invitrogen, Carlsbad, CA)according to manufacturer's protocol.
Label Biotin
Label protocol Target was labeled with Enzo BioArray High Yield RNA Trascript Labeling Kit (Enzo Life Technologies, Farmingdale, NY) according to manufacturer's protocol.
 
Hybridization protocol Hybridization was performed according to Affymetrix protocol.
Scan protocol Arrays were scanned according to Affymetrix protocol.
Description GSM240432
4PB_27_M_DON040
Cryopreserved MNCs
Data processing .DAT files were generated using GCOS 1.2.1 software (Affymetrix). CEL files were generated using MAS 5.0 softare (Affymetrix) with target signals for probe sets scaled to 500. Model-based expression levels were computed from .CEL files using dChip software after normalization by invariant set method.
 
Submission date Apr 16, 2012
Last update date Apr 17, 2012
Contact name Sebastian K Ochsenreither
Organization name Charite Berlin
Department Hematology/Oncology
Street address Hindenburgdamm 30
City Berlin
State/province Berlin
ZIP/Postal code 12203
Country Germany
 
Platform ID GPL96
Series (1)
GSE37307 Aberrant expressed genes in AML
Relations
Reanalysis of GSM240432

Data table header descriptions
ID_REF
VALUE linear normalized signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 329.13
AFFX-BioB-M_at 412.49
AFFX-BioB-3_at 391.8
AFFX-BioC-5_at 658.55
AFFX-BioC-3_at 855.31
AFFX-BioDn-5_at 1323.91
AFFX-BioDn-3_at 3691.38
AFFX-CreX-5_at 7766.66
AFFX-CreX-3_at 8384.64
AFFX-DapX-5_at 17.08
AFFX-DapX-M_at 23.9
AFFX-DapX-3_at 10.74
AFFX-LysX-5_at 3.07
AFFX-LysX-M_at 8.65
AFFX-LysX-3_at 7.55
AFFX-PheX-5_at 3.46
AFFX-PheX-M_at 8
AFFX-PheX-3_at 70.11
AFFX-ThrX-5_at 7.56
AFFX-ThrX-M_at 14.26

Total number of rows: 22283

Table truncated, full table size 370 Kbytes.




Supplementary file Size Download File type/resource
GSM915764_4PB_27_M_DON040.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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