|
Status |
Public on Apr 17, 2012 |
Title |
FHCRCAML1355 |
Sample type |
RNA |
|
|
Source name |
leukemia
|
Organism |
Homo sapiens |
Characteristics |
diagnosis: AML cell type: Cryopreserved MNCs
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using TRIzol reagent (Invitrogen, Carlsbad, CA)according to manufacturer's protocol.
|
Label |
Biotin
|
Label protocol |
Target was labeled with Enzo BioArray High Yield RNA Trascript Labeling Kit (Enzo Life Technologies, Farmingdale, NY) according to manufacturer's protocol.
|
|
|
Hybridization protocol |
Hybridization was performed according to Affymetrix protocol.
|
Scan protocol |
Arrays were scanned according to Affymetrix protocol.
|
Description |
GSM239371 6AML_34P_D_68F_1_BM_I_1355 Cryopreserved MNCs
|
Data processing |
.DAT files were generated using GCOS 1.2.1 software (Affymetrix). CEL files were generated using MAS 5.0 softare (Affymetrix) with target signals for probe sets scaled to 500. Model-based expression levels were computed from .CEL files using dChip software after normalization by invariant set method.
|
|
|
Submission date |
Apr 16, 2012 |
Last update date |
Apr 17, 2012 |
Contact name |
Sebastian K Ochsenreither |
Organization name |
Charite Berlin
|
Department |
Hematology/Oncology
|
Street address |
Hindenburgdamm 30
|
City |
Berlin |
State/province |
Berlin |
ZIP/Postal code |
12203 |
Country |
Germany |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE37307 |
Aberrant expressed genes in AML |
|
Relations |
Reanalysis of |
GSM239371 |