|
| Status |
Public on Apr 17, 2012 |
| Title |
FHCRCAML2619 |
| Sample type |
RNA |
| |
|
| Source name |
leukemia
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: AML
cell type: Cryopreserved MNCs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using TRIzol reagent (Invitrogen, Carlsbad, CA)according to manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Target was labeled with Enzo BioArray High Yield RNA Trascript Labeling Kit (Enzo Life Technologies, Farmingdale, NY) according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to Affymetrix protocol.
|
| Scan protocol |
Arrays were scanned according to Affymetrix protocol.
|
| Description |
GSM239489
7AML_34N_D_53M_4_BM_N_2619
Cryopreserved MNCs
|
| Data processing |
.DAT files were generated using GCOS 1.2.1 software (Affymetrix). CEL files were generated using MAS 5.0 softare (Affymetrix) with target signals for probe sets scaled to 500. Model-based expression levels were computed from .CEL files using dChip software after normalization by invariant set method.
|
| |
|
| Submission date |
Apr 16, 2012 |
| Last update date |
Apr 17, 2012 |
| Contact name |
Sebastian K Ochsenreither |
| Organization name |
Charite Berlin
|
| Department |
Hematology/Oncology
|
| Street address |
Hindenburgdamm 30
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
12203 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE37307 |
Aberrant expressed genes in AML |
|
| Relations |
| Reanalysis of |
GSM239489 |
