|
| Status |
Public on Dec 10, 2012 |
| Title |
Healthy Living donor [H7-Glom-LD978] |
| Sample type |
RNA |
| |
|
| Source name |
Kidney Biopsy from Human Healthy Living donor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Glomeruli from kidney biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Rneasy kit (Qiagen) following manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
According to the Affymetrix Expression Analysis Technical Manual.
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression data from the glomeruli of human pre-transplant living donor control kidney
|
| Data processing |
The data were analyzed using GenePattern pipeline: RMA method and Human Entrez Gene custom CDF annotation version 10 was used for the normalization
Each of the hybridization was normalized seperately and the VALUE column contains the batch-corrected data using Combat
|
| |
|
| Submission date |
Apr 20, 2012 |
| Last update date |
Dec 11, 2012 |
| Contact name |
Viji Nair |
| E-mail(s) |
vijin@med.umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1152 West Medical Centre Dr.
|
| City |
Ann Arbor |
| ZIP/Postal code |
48331 |
| Country |
USA |
| |
|
| Platform ID |
GPL11670 |
| Series (2) |
| GSE37460 |
Cross-species transcriptional network analysis defines shared inflammatory responses in murine and human lupus nephritis [Glomeruli] |
| GSE37463 |
Cross-species transcriptional network analysis defines shared inflammatory responses in murine and human lupus nephritis |
|
