|
| Status |
Public on Mar 30, 2013 |
| Title |
SCI-ES-3Days-3 |
| Sample type |
RNA |
| |
|
| Source name |
soleus
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: Female
spine surgery: SCI
electrical stimulation (es): Yes
gastrocnemius ablation (ga): Yes
time after es/ga (days): 3
tissue: soleus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pieces of muscle weighing approximately 20 g were homogenized in Trizol reagent. Total RNA was then extracted following the manufacturers recommended procedures and further purified on RNAeasy columns (Qiagen) then quantified by absorbance at 260 nm. RNA integrity numbers were greater than 8.0 for all samples.
|
| Label |
biotin
|
| Label protocol |
according the the manufacturers recommended procedures
|
| |
|
| Hybridization protocol |
according the the manufacturers recommended procedures
|
| Scan protocol |
according the the manufacturers recommended procedures
|
| Description |
Sample name: 1299
|
| Data processing |
Preprocessing was performed using the RMA function of the Oligo package for Bioconductor.
|
| |
|
| Submission date |
Apr 21, 2012 |
| Last update date |
Mar 30, 2013 |
| Contact name |
christopher p cardozo |
| E-mail(s) |
chris.cardozo@mssm.edu
|
| Phone |
718-584-9000
|
| Organization name |
Bronx VA Medical Center
|
| Department |
CMSCI
|
| Lab |
Room 3F-35
|
| Street address |
130 West Kingsbridge Road
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10468 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE37476 |
Time course of gene expression changes after muscle contraction in spinal cord injured rats |
|
