|
| Status |
Public on Aug 07, 2012 |
| Title |
WT leaf |
| Sample type |
SRA |
| |
|
| Source name |
leaves
|
| Organism |
Zea mays |
| Characteristics |
tissue: leaf
developmental stage: 3 weeks after germination
genotype: wild type (B73)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using TRIzol reagent (Invitrogen). 16-30 nt small RNAs were gel purified from 15% PAGE gel, 5' and 3' adaptors were added and followed by RT-PCR using adaptor-specific primers. The PCR products were isolated and purified from agarose gel and used for cluster generation
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
Illumina Casava1.4 for basecalling.
Read-count were generated after filtering of adaptor sequences and removal of low quality reads by Illumina's GA pipeline.
Genome_build: RefGen_v2(5b.60)
Supplementary_files_format_and_content: tab-delimited text files containing length, read count and sequence
|
| |
|
| Submission date |
Apr 24, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Mingming Kang |
| E-mail(s) |
kanglmf@gmail.com
|
| Organization name |
China Agricultural University
|
| Street address |
No. 2 Yuanmingyuan West Rd,
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL9141 |
| Series (1) |
| GSE37551 |
Characterization of microRNAs expression during maize seed development |
|
| Relations |
| SRA |
SRX144280 |
| BioSample |
SAMN00862618 |
