|
| Status |
Public on Dec 31, 2012 |
| Title |
muscle O2 3% vs. muscle O2 21% array 5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Spalax muscle 3% oxygen
|
| Organism |
Nannospalax galili |
| Characteristics |
tissue: muscle
treatment: O2 3%
time: 6h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with Trizol
|
| Label |
Cy5
|
| Label protocol |
Agilent low input 2-color amplification kit
|
| |
|
| Channel 2 |
| Source name |
Spalax muscle 21% oxygen
|
| Organism |
Nannospalax galili |
| Characteristics |
tissue: muscle
treatment: O2 21%
time: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with Trizol
|
| Label |
Cy3
|
| Label protocol |
Agilent low input 2-color amplification kit
|
| |
|
| |
| Hybridization protocol |
Agilent hybridization protocol
|
| Scan protocol |
Axon 4000B GenePix 6.1
|
| Data processing |
Feature Extraction software- GenePix 6.1
Data from each slide was background corrected and Lowess normalized
|
| |
|
| Submission date |
Apr 25, 2012 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Mark Robert Band |
| E-mail(s) |
markband@uiuc.edu
|
| Phone |
217 244 3930
|
| Fax |
217 265 5066
|
| Organization name |
University of Illinois
|
| Department |
Biotechnology
|
| Lab |
ERML 356
|
| Street address |
1201 W. Gregory Dr
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platform ID |
GPL15478 |
| Series (1) |
| GSE37619 |
Spalax gene expression profiling of muscle and brain under hypoxia |
|
